Expression of MC4R and AgRP mRNAs in Arcuate Nucleus (ARC)

Expression of MC4R and AgRP mRNAs in Arcuate Nucleus (ARC) Expression of melanocortin-4 receptor and agouti-related peptide mRNAs in arcuate nucleus during long term malnutrition of female ovariectomized rats Abstract Objective: Melanocortin-4 receptor (MC4R) and agouti-related peptide (AgRP) are involved in energy homeostasis in the rat. The aim of the present study was to evaluate the expression of MC4R and AgRP mRNAs in arcuate nucleus (ARC) during long term malnutrition of female ovariectomized rats. Materials and Methods: Twelve female ovariectomized rats were divided into two equal groups (n=6) of normal and restricted diet groups. Four ovariectomized rats were selected as controls. Using real-time PCR, the relative expressions (compared to controls) of MC4R and AgRP mRNAs in the ARC of rats were compared between both diet groups. Results: The relative expression of MC4R and AgRP mRNA in the ARC of female ovariectomized rats during long term malnutrition was higher than those with normal diet (P